SYBR® Safe is a DNA stain that glows bright green when exposed to UV or blue-light.
- Safe for biotechnology classrooms
- More sensitive than ethidium bromide
- Non-mutagenic
Like ethidium bromide, SYBR® Safe binds specifically to the DNA double helix. Unlike ethidium bromide, SYBR® Safe is less mutagenic and more sensitive, making it safer to use, particularly in the classroom.
To pre-stain your gel before you perform electrophoresis: use 5 µl of the bulk stock for every 50 ml of molten agarose.
To use as a post-stain after your gel has been run: use 7.5 ul of the bulk stock for every 75 ml of electrophoresis buffer.
Cover staining tray with foil and let sit for 10 to 15 minutes.